The collection of games contains optimized versions of the 3D Mario games Super Mario 64, Super Mario Sunshine and Super Mario Galaxy. In addition to having higher resolutions than their original versions, the games have been optimized for a smooth gameplay experience on Nintendo Switch.

Super Mario 3D All-Stars also includes an in-game music-player mode to play the music and songs from all three games. Players can also listen to music on their Nintendo Switch systems when the screen is off.